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der diagnostischen Prestatien der kommerziellen RT-PCR-Kits für die Detektion

der diagnostischen Prestatien der kommerziellen RT-PCR-Kits für die Detektion

Von Mai 25, 2021 Chlamydia, E. coli, Gene, Polyclonal, Recombinant, Serum, Western Blot Keine Kommentare

Analysis of the diagnostic prestatien of the industrial RT-PCR kits for the detection of SARS-CoV-2 in Colombia

The ongoing SARS-CoV-2 pandemic has led to the design and development of several RT-PCR kits designed to enable rapid scale-up of molecular exams for massive screening. We evaluated the diagnostic performance of nine commercial kits that showed optimal performance and high discriminatory power. However, we observed differences in sensitivity, specificity and E-Gen-Ct values ​​and discussed these results in light of the influence of  the  genetic variability  of SARS-CoV-2  and its possible impact on current molecular diagnostic assays. This article is protected by copyright. All rights reserved.

Antimikrobielle Aktivität von Kamferboomzilvernanodeeltjes tegen vuilbroedziekten en het ontdekken van een nieuwe stam van  Serratia marcescens mittels DGGE-PCR  als Sekundärinfektion auf Honingbijlarven verabreicht wurde

Amerikanische Faulbrut (AFB) und Europäische Faulbrut (EFB) sind die beiden wichtigsten bakteriellen Krankheiten, von denen Honigbienen betroffen sind. Dies führt zu einer Verringerung der Lebensfähigkeit des Bienenstocks, einer Verringerung der Honigproduktion und zu erheblichen wirtschaftlichen Verlusten für die Imker.

Aufgrund der Ineffizienz und / oder geringen Wirksamkeit einiger Antibiotika stellen Forschungen mit Nanotechnologie möglicherweise neue therapeutische Strategien dar. Nanostruktur-Medikamente haben gegenüber herkömmlichen Medikamenten einige Vorteile gezeigt , wie langsame, schrittweise und kontrollierte Freisetzung, erhöhte Bioverfügbarkeit und verringerte Nebenwirkungen.

In dieser Studie wurden verschiedene infizierte Larven aus zwei Bienenhäusern gesammelt; Die Kämme, die Symptome einer amerikanischen und europäischen Faulbrut aufwiesen, wurden isoliert.

Die  antimikrobielle In-vitro- Aktivität von Kampferbaum-Silber-Nanopartikeln gegen Faulbrutkrankheiten wurde unter Verwendung von UV-Vis-Spektrophotometrie und Rasterelektronenmikroskop (SEM) charakterisiert , was die Bildung von Silbernanopartikeln mit einem Größenbereich von 160 bis 660 nm belegt. Die antimikrobielle Aktivität der Silbernanopartikel wurde unter Verwendung eines Agardiffusionsassays getestet und bewies ihre Fähigkeit, das pathogene Bakterienwachstum sowohl in AFB als auch in EFB wirksam zu stoppen .

Die DGGE-PCR-Technik wurde zur Identifizierung seltener bakterieller Infektionen von Honigbienen in Abhängigkeit von der 16S-rRNA-Amplifikation aus ihrer gesamten extrahierten DNA angewendet und als Serratia marcescens  (TES) identifiziert  , die in der GenBank mit einer neuen Zugangsnummer (MT240613) hinterlegt ist.

Die Ergebnisse bestätigten, dass der Stamm durch DGGE-PCR-Analyse nachgewiesen wurde, die eine einzigartig infizierte Brut verursachte, die von der amerikanischen Foulbrood angegriffen wurde. Es konnte gefolgert werden, dass grün synthetisierte Silbernanopartikel voraussichtlich als wirksame Behandlung für bakterielle Honigbienenkrankheiten eingesetzt werden. Diese Materialien müssen unter Feldbedingungen genauer untersucht werden und untersuchen die Möglichkeit ihrer Rückstände in Honigbienen wie Honig und Bienenwachs.

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Recombinant Human Relaxin-3
MBS140915-1mg 1mg
EUR 2880

Recombinant Human Relaxin-3
MBS140915-5x1mg 5x1mg
EUR 12630

Relaxin-3, human recombinant
7195-10 each
EUR 248.4

Relaxin-3, human recombinant
7195-50 each
EUR 810

Relaxin-3 Human Recombinant
rAP-4650 Inquiry Ask for price

INSL7 / Relaxin 3 (Human)
035-36 20 μg
EUR 160.92

INSL7 / Relaxin 3 (Human)
035-36A 100 μg
EUR 604.8

Human Relaxin-3,RLN-3 ELISA
YLA4309HU-48T 48T Ask for price

Human Relaxin-3,RLN-3 ELISA
YLA4309HU-96T 96T Ask for price

Human Relaxin-3 ELISA Kit
MBS765502-10x96StripWells 10x96-Strip-Wells
EUR 3900

Human Relaxin-3 ELISA Kit
MBS765502-48StripWells 48-Strip-Wells
EUR 340

Human Relaxin-3 ELISA Kit
MBS765502-5x96StripWells 5x96-Strip-Wells
EUR 2045

Human Relaxin-3 ELISA Kit
MBS765502-96StripWells 96-Strip-Wells
EUR 455

Human Relaxin-3 ELISA Kit
MBS2887639-10x96StripWells 10x96-Strip-Wells
EUR 4080

Human Relaxin-3 ELISA Kit
MBS2887639-48StripWells 48-Strip-Wells
EUR 390

Human Relaxin-3 ELISA Kit
MBS2887639-5x96StripWells 5x96-Strip-Wells
EUR 2220

Human Relaxin-3 ELISA Kit
MBS2887639-96StripWells 96-Strip-Wells
EUR 520

INSL7 / Relaxin 3 (Human) - Antibody
H-035-36 50 μl
EUR 604.8

Mouse Monoclonal anti-human Relaxin-3
hAP-0526 100ug
EUR 250

Human Relaxin 3 ELISA Kit
EK5653 96 tests
EUR 599

Human Relaxin 3 ELISA Kit
MBS054925-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Relaxin 3 ELISA Kit
MBS054925-48StripWells 48-Strip-Wells
EUR 550

Human Relaxin 3 ELISA Kit
MBS054925-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Relaxin 3 ELISA Kit
MBS054925-96StripWells 96-Strip-Wells
EUR 765

Human relaxin 3 ELISA Kit
SL2396Hu 96 Tests
EUR 468

Human Relaxin 3 ELISA Kit
MBS8248392-5x96Tests 5x96Tests
EUR 2880

Human Relaxin 3 ELISA Kit
MBS8248392-96Tests 96Tests
EUR 645

Human Relaxin-3 Recombinant Protein
100-356 25 µg
EUR 196.35
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Human Relaxin-3 Recombinant Protein
100-356S 5 µg
EUR 92.4
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Recombinant Human Relaxin-3 Protein
PROTQ8WXF3-1 25ug
EUR 380.4
Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain.

Human Relaxin 3 (RLN3) Protein
abx680520-100g 100 µg
EUR 225

Human Relaxin-3 (RLN3) ELISA Kit
EK7514 96Т
EUR 799

Human Relaxin-3(RLN3) Elisa Kit
EK713553 96 Wells
EUR 0.37

Human Relaxin-3 (RLN3) ELISA Kit
AE22052HU-48Tests 48 Tests
EUR 325
Description: Human (Homo sapiens)

Human Relaxin-3 (RLN3) ELISA Kit
AE22052HU-96Tests 96 Tests
EUR 610
Description: Human (Homo sapiens)

Human Relaxin-3 (RLN3) ELISA Kit
AE22052HU-48T 48T
EUR 325
Description: Human (Homo sapiens)

Human Relaxin-3 (RLN3) ELISA Kit
AE22052HU-96T 96T
EUR 610
Description: Human (Homo sapiens)

Human Relaxin-3 (RLN3) ELISA Kit
KTE60790-48T 48T
EUR 339
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3.

Human Relaxin-3 (RLN3) ELISA Kit
KTE60790-96T 96T
EUR 589
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3.

Human Relaxin-3 (RLN3) ELISA Kit
KTE60790-96T5 96 T×5
EUR 2629
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3.

Human Relaxin-3 (RLN3) ELISA Kit
KTE60790-96T50 96 T×50
EUR 24569
Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3.

Human Relaxin-3 (RLN3) ELISA Kit
RK06347 96T
EUR 280

Human Relaxin-3 (RLN3) ELISA Kit
MBS9714455-48Tests 48Tests
EUR 340

Human Relaxin-3 (RLN3) ELISA Kit
MBS9714455-5x96Tests 5x96Tests
EUR 1820

Human Relaxin-3 (RLN3) ELISA Kit
MBS9714455-96Tests 96Tests
EUR 500

Human Relaxin-1
hAP-0525 50ug
EUR 400

Human Relaxin-2
MBS691734-0005mg 0.005mg
EUR 300

Human Relaxin-2
MBS691734-0025mg 0.025mg
EUR 450

Human Relaxin-2
MBS691734-5x0025mg 5x0.025mg
EUR 1725

Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7)
MBS650130-0005mg 0.005mg
EUR 340

Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7)
MBS650130-0025mg 0.025mg
EUR 490

Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7)
MBS650130-5x0025mg 5x0.025mg
EUR 2055

Relaxin-2 (Human)
MBS408061-005mg 0.05mg
EUR 345

Relaxin-2 (Human)
MBS408061-5x005mg 5x0.05mg
EUR 1500

Human RLN3(Relaxin-3) ELISA Kit
EH0755 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human RLN3/ Relaxin-3 ELISA Kit
E2158Hu 1 Kit
EUR 515
Description: UNQ6188/PRO20213,INSL7,RXN3,ZINS4,Insulin-like peptide INSL7,Prorelaxin H3,Insulin-like peptide 7

Human RLN3 / Relaxin-3 ELISA Kit
E0189h 96T
EUR 736

Human RLN3(Relaxin-3) ELISA Kit
RE1961H-48wells 48 wells
EUR 125.55

Human RLN3(Relaxin-3) ELISA Kit
RE1961H-96wells 96 wells
EUR 179.55

Human RLN3 (Relaxin-3) ELISA Kit
MBS2516020-10x96Tests 10x96Tests
EUR 4060

Human RLN3 (Relaxin-3) ELISA Kit
MBS2516020-24Tests 24Tests
EUR 265

Human RLN3 (Relaxin-3) ELISA Kit
MBS2516020-48Test 48Test
EUR 450

Human RLN3 (Relaxin-3) ELISA Kit
MBS2516020-5x96Test 5x96Test
EUR 2265

Human RLN3 (Relaxin-3) ELISA Kit
MBS2516020-96Tests 96Tests
EUR 535

Human RLN3(Relaxin-3) ELISA Kit
RE1961H-48wellsplate 48 wells plate
EUR 126

Human RLN3(Relaxin-3) ELISA Kit
RE1961H-96wellsplate 96 wells plate
EUR 180

Human Relaxin 3 (RLN3) ELISA Kit
abx253713-96tests 96 tests
EUR 904.8

Human Relaxin 3 (RLN3) ELISA Kit
abx512123-96tests 96 tests
EUR 904.8

Human Relaxin 3,RLN3 ELISA KIT
E7543Hu-1096T 10*96T
EUR 4122

Human Relaxin 3,RLN3 ELISA KIT
E7543Hu-48wells 48 wells
EUR 300

Human Relaxin 3,RLN3 ELISA KIT
E7543Hu-596T 5*96T
EUR 2061

Human Relaxin 3,RLN3 ELISA KIT
E7543Hu-96wells 96 wells
EUR 458

Human Relaxin 3(RLN3)ELISA Kit
QY-E01376 96T
EUR 433.2

Human Relaxin 3, RLN3 ELISA Kit
MBS1609027-10x96StripWells 10x96-Strip-Wells
EUR 3460

Human Relaxin 3, RLN3 ELISA Kit
MBS1609027-48StripWells 48-Strip-Wells
EUR 285

Human Relaxin 3, RLN3 ELISA Kit
MBS1609027-5x96StripWells 5x96-Strip-Wells
EUR 1750

Human Relaxin 3, RLN3 ELISA Kit
MBS1609027-96StripWells 96-Strip-Wells
EUR 425

Nori® Human Relaxin-3 ELISA Kit
GR111288 96-well
EUR 461

Recombinant Human Relaxin-3 (RLN3), partial
MBS7094488-005mgBaculovirus 0.05mg(Baculovirus)
EUR 885

Recombinant Human Relaxin-3 (RLN3), partial
MBS7094488-005mgEColi 0.05mg(E-Coli)
EUR 510

Recombinant Human Relaxin-3 (RLN3), partial
MBS7094488-02mgEColi 0.2mg(E-Coli)
EUR 665

Recombinant Human Relaxin-3 (RLN3), partial
MBS7094488-02mgYeast 0.2mg(Yeast)
EUR 945

Recombinant Human Relaxin-3 (RLN3), partial
MBS7094488-05mgEColi 0.5mg(E-Coli)
EUR 710

ELISA kit for Human Relaxin-3 (RLN3)
KTE60790-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

INSL7 / Relaxin 3 (Human) - RIA Kit
RK-035-36 125 tubes
EUR 1061.64

Relaxin-3
AP87248 1mg
EUR 2640

Relaxin-3
AP87370 1mg
EUR 2640

Relaxin-3
AP87734 1mg
EUR 2640

Relaxin-3
AP88315 1mg
EUR 2640

Relaxin H3 (human)
4039569.02 0.2 mg
EUR 455.07

Relaxin H3 (human)
4039569.1 1 mg
EUR 1818.92

Relaxin H2 (human)
4039910.02 0.2 mg
EUR 455.07

Relaxin H2 (human)
4039910.1 1 mg
EUR 1818.92

Relaxin H2 (human)
H-6784.0200 0.2mg
EUR 741.6
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H2 (human)
H-6784.1000 1.0mg
EUR 2853.6
Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net

Relaxin H3 (human)
H-7322.0200 0.2mg
EUR 741.6
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Relaxin H3 (human)
H-7322.1000 1.0mg
EUR 2853.6
Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net

Relaxin 2 (Human)
035-62 20 μg
EUR 261.36

Relaxin 2 (Human)
035-62A 100 μg
EUR 469.8

Human RLN3(Relaxin 3) ELISA Kit
ELK9324-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RLN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RLN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RLN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RLN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human RLN3(Relaxin 3) ELISA Kit
ELK9324-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RLN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RLN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RLN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RLN3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human RLN3 (Relaxin 3) ELISA Kit
MBS8819812-10x96StripWells 10x96-Strip-Wells
EUR 3130

Human RLN3 (Relaxin 3) ELISA Kit
MBS8819812-48StripWells 48-Strip-Wells
EUR 350

Human RLN3 (Relaxin 3) ELISA Kit
MBS8819812-5x96StripWells 5x96-Strip-Wells
EUR 1710

Human RLN3 (Relaxin 3) ELISA Kit
MBS8819812-96StripWells 96-Strip-Wells
EUR 445

Anti- Relaxin-3 receptor 2 Antibody
GWB-214500 0.05 ml Ask for price

Anti- Relaxin-3 receptor 2 Antibody
GWB-EDDDF9 0.05 ml Ask for price

Relaxin 2 (Human) - Antibody
H-035-62 100 μl
EUR 604.8

Human Relaxin 3 PicoKine ELISA Kit
EK1407 96 wells
EUR 510
Description: For quantitative detection of human Relaxin 3 in cell culture supernates, serum and plasma( heparin).

RLN3 (untagged)-Human relaxin 3 (RLN3)
SC305868 10 µg Ask for price

Human Relaxin 3 PicoKine ELISA Kit
MBS177627-5x96StripWells 5x96-Strip-Wells
EUR 3935

Human Relaxin 3 PicoKine ELISA Kit
MBS177627-96StripWells 96-Strip-Wells
EUR 855

ELISA kit for Human RLN3 (Relaxin-3)
E-EL-H1652 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human RLN3 (Relaxin-3) in samples from Serum, Plasma, Cell supernatant

Relaxin 3 (BDelta23-27) R (Human)
036-20 100 μg
EUR 461.16

RLN3 Relaxin-3 Human Recombinant Protein
PROTQ8WXF3 Regular: 25ug
EUR 380.4
Description: Relaxin-3 Human Recombinant produced in E.Coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain.;The Relaxin-3 is purified by proprietary chromatographic techniques.

INSL7 / Relaxin 3 (Human) - Cy5 Labeled
FC5-035-36 1 nmol
EUR 756

INSL7 / Relaxin 3 (Human) - Purified IgG Antibody
G-035-36 200 μg
EUR 604.8

Human Relaxin-3 Recombinant Protein Lyophilized
IHURLXN3RLY5UG each
EUR 341
Description: Human Relaxin-3 Recombinant Protein Lyophilized

Human Relaxin-3 Recombinant Protein Lyophilized
MBS8433281-INQUIRE INQUIRE Ask for price

Recombinant Human Relaxin-2
MBS146516-0005mg 0.005mg
EUR 240

Een zeer gevoelige en specifieke sonde-gebaseerde Echtzeit-PCR für den Nachweis von  Avibacterium paragallinarum  in klinischen Monstern van pluimvee

Avibacterium paragallinarum  (historisch genannt  Haemophilus paragallinarum ) verursacht infektiöse Coryza (IC), die eine akute respiratorische Erkrankung von Hühnern ist. Kürzlich wurden in Pennsylvania (PA) Ausbrüche von IC bei Broilern, Legehennen und Legehennen gemeldet, die zu erheblichen Atemwegserkrankungen und Produktionsverlusten führten. Eine vorläufige Diagnose des IC kann auf der Grundlage der Anamnese, der klinischen Symptome und der charakteristischen groben Läsionen gestellt werden. Für eine endgültige Diagnose sind jedoch eine Isolierung und Identifizierung des Organismus erforderlich. Große Herausforderungen bei der bakteriologischen Diagnose von  A. paragallinarum,  gehört, dass der Organismus schwer zu isolieren ist, langsam wächst und nur erfolgreich sein kann Im akuten Stadium der Infektion isolierte und sekundäre bakterielle Infektionen sind ebenfalls häufig.

Da  die öffentlichen Datenbanken nur sehr begrenzte Gesamtgenome von  A. paragallinarum enthielten , führten wir eine Gesamtgenomsequenzierung (WGS) von PA-Isolaten durch und basierten auf der WGS-Datenanalyse. Wir haben einen neuartigen sondenbasierten PCR-Assay entwickelt, der auf eine hochkonservierte Sequenz im  recN , dem DNA-Reparaturprotein-Gen von  A. paragallinarum, abzielt . Der Assay umfasst eine interne Kontrolle mit einer Nachweisgrenze (LOD) von 3,93 genomischen Kopien. Die PCR-Effizienz lag zwischen 90 und 97% und die diagnostische Sensitivität von 98,5% im Vergleich zur herkömmlichen gelbasierten PCR.

Der Take a look at battle hochspezifisch und es wurde keine Kreuzreaktivität mit anderen Avibacterium- Arten   und einer Reihe anderer üblicher viraler und bakterieller Pathogene der Atemwege von Geflügel beobachtet . Echtzeit-PCR-Exams an 419 klinischen Proben aus vermuteten Herden ergaben 94 optimistic und 365 adverse Ergebnisse in Übereinstimmung mit dem diagnostischen Nachweis auf der Foundation von Bakterienkulturen. Wir verglichen auch den  recN-  PCR- Assay mit einem früheren  HPG-2-  basierten Echtzeit-PCR-Assay, der eine PCR-Effizienz von 79% zeigte.

Gelijktijdige detectie en differentiatie van klinisch relevante recidiverende koorts  Borrelia  erfüllt semi-Multiplex real-time PCR

 

Ailments transmitted by bacterial vectors, together with   Borrelia   species, pose a big diagnostic, medical, and well being problem due to their overlapping signs and the breadth of pathogens and arthropod vectors.   Recurrent  fever borrelia (RF) consists of  each established and rising pathogens are transmitted to people by gentle ticks, onerous ticks or lice. 

We’ve developed a real-time, semi-multiplexed PCR assay that detects a number of RF borrelia, which trigger illness in people, and divides them into one among three teams. 

The teams are primarily based on genetic similarity and embody pathogens inflicting relapsing fever in gentle ticks (  B. hermsii   and others), the rising onerous tick-borne pathogen   B. miyamotoi, and the pathogen accountable for relapsing fever  (  B. recentis)   . Assay makes use of a single primer pair designed to amplify all recognized RF pathogenic borrelia and a number of TaqMan probes to allow detection and differentiation between the three teams.

The assay acknowledges all examined RF Borrelia with an analytical detection restrict of lower than 15 genome equivalents per response. Thirty isolates of  RF Borrelia spanning  six species have been precisely recognized. 39 of 41 residual samples  (EDTA entire blood  , serum or plasma) from sufferers with RF had been detected and accurately categorized. 

None of 42 medical specimens from sufferers with different infections and 46 tradition specimens from non-RF micro organism had been detected. Creating a real-time PCR method to a single assay will assist  enhance  the prognosis of  RF by simplifying the choice  of assessments   to help the medical administration of acutely unwell RF sufferers.

Overlevingsanalyse van de tijd tot SARS-CoV-2 PCR-Negativierung om PCR-Receptor bij gezondheidswerkers te optimaliseren: de Henares COVID-19 cohort research voor gezondheidswerkers

Goals:   Reverse transcriptase  PCR (RT-PCR)  is taken into account the gold commonplace in diagnosing COVID-19. Contaminated well being care staff won’t return to work till RT-PCR exhibits that the virus is now not current within the higher respiratory tract. The goal of this research is to find out essentially the most environment friendly time to carry out an RT-PCR previous to reintegrating healthcare staff.

Supplies and Strategies:   This can be a cohort research of  staff  of well being care  with RT-PCR confirmed  COVID-19th The info was collected from the well being care staff‘ medical charts and accomplished with a phone interview. Kaplan-Meier curves had been used to find out the affect of a number of variables on the time to RT-PCR negatization. The affect of the variables on survival was assessed utilizing the Breslow check. A Cox regression mannequin was developed containing the related variables.

Outcomes:   159 topics with a optimistic  RT-PCR from 374  staff with suspected COVID-19 had been included. The median time to negatization was 25 days from the onset of signs (IQR 20-35 days). The presence of IgG, dyspnoea, cough, and sore throat had been related to a considerably longer time to negatization. Cox logistic regression was used to regulate for complicated variables. Solely dyspnoea and cough remained within the mannequin as important determinants for a chronic negativity time. The adjusted HRs had been 0.68 (0.48-096) for dyspnea  and 0.61 (0.42-0.88)  for dry cough.

Conclusions:   RT-PCR within the first Three weeks results in a excessive share of optimistic outcomes. If there have been respiratory issues, the negatization lasted virtually per week longer. Those that developed antibodies took longer to negate.

SYBR Green qPCR Master Mix (No ROX)
C0006-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (No ROX)

SYBR Green qPCR Master Mix (No ROX)
HY-K0523 5 mL (500 rxns )
EUR 308.4

SYBR Green qPCR Master Mix (Low ROX)
C0008-10mg 10mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-1g 1g Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-1mg 1mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
C0008-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (Low ROX)

SYBR Green qPCR Master Mix (Low ROX)
HY-K0522 5 mL (500 rxns )
EUR 308.4

SYBR Green qPCR Master Mix (High ROX)
C0007-10mg 10mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-1g 1g Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-1mg 1mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-50mg 50mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
C0007-5mg 5mg Ask for price
Description: SYBR Green qPCR Master Mix (High ROX)

SYBR Green qPCR Master Mix (High ROX)
HY-K0521 1 mL (100 rxns)
EUR 135.6

2x SYBR Green qPCR Master Mix
B21202 5 mL
EUR 268.8
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

2x SYBR Green qPCR Master Mix
B21203 25 mL
EUR 1027.2
Description: Our 2x SYBR Green qPCR master mix performs toe to toe in all qPCR assays with the most well known brands on the market but surpases them significantly in cost-efficiency.

SYBR Green qPCR Master Mix
HY-K0501 5 mL (500 rxns)
EUR 315.6

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-25 25ml
EUR 336

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-5 5ml
EUR 96

HotStart™ 2X SYBR Green qPCR Master Mix
K1070-50 50ml
EUR 672

ig SYBR Green qPCR 2X Master Mix - 200 Reactions
3354 200
EUR 110
Description: Intact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-25 25ml
EUR 336
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-5 5ml
EUR 96
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

HotStart™ Universal 2X SYBR Green qPCR Master Mix
K1170-50 50ml
EUR 672
Description: qPCR Kit, Universal SYBR Green I Master Mix (Rox)

Maxima SYBR Green/ROX qPCR Master Mix - 200 x 25 µL rxns
THK0221 each Ask for price

Maxima SYBR Green/ROX qPCR Master Mix - 1,000 x 25 µL rxns
THK0222 each Ask for price

Maxima SYBR Green/ROX qPCR Master Mix - 4,000 x 25 µL rxns
THK0223 each
EUR 3324.5

AceQ qPCR SYBR® Green Master Mix
Q111-02 500 rxn (20 μl/rxn)
EUR 265.2

AceQ qPCR SYBR® Green Master Mix
Q111-03 2500 rxn (20 μl/rxn)
EUR 775.2

2X SYBR Green Fast qPCR Mix with High ROX
RK21201 50mL
EUR 109

ViPrimePLUS Taq qPCR Green Master Mix I with ROX, 150rxns
QLMM12-R each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I with ROX, 150rxns
QLMM02-R each Ask for price

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix (Without ROX)
Q321-03-2500rxns 2,500 rxns
EUR 562.79

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 200 x 25 µL rxns
THK0251 each
EUR 232.17

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 1,000 x 25 µL rxns
THK0252 each
EUR 1046.4

Maxima SYBR Green qPCR Master Mix, ROX Solution provided - 4,000 x 25 µL rxns
THK0253 each Ask for price

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17270 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17270-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17271 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17271-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17272 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17272-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17273 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17273-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17274 1 mL
EUR 203
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17274-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17275 5 mL
EUR 508
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17275-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

BioEasy Master Mix (SYBR Green, No ROX)
TRI-B25L1D 200T
EUR 403.2

ViPrimePLUS Taq qPCR Green Master Mix I with Low ROX, 150rxns
QLMM12-LR each Ask for price

BioEasy Master Mix (SYBR Green, low ROX)
TRI-B25L1C 200T
EUR 403.2

BioEasy Master Mix (SYBR Green, high ROX)
TRI-B25L1B 200T
EUR 403.2

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix (Without ROX)
Q421-03-2500rxns 2,500 rxns
EUR 656.51

ViPrimePLUS AtTaq qPCR Green Master Mix I with Low ROX, 150rxns
QLMM02-LR each Ask for price

ViPrimePLUS Taq qPCR Green Master Mix II with ROX, 150rxns
QLMM17-R each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II with ROX, 150rxns
QLMM07-R each Ask for price

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17276 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17276-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17277 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *No ROX*
17277-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17278 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17278-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17279 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *Low ROX*
17279-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17280 1 mL
EUR 203
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17280-1mL 1 mL
EUR 99
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17281 5 mL
EUR 508
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green *High ROX*
17281-5mL 5 mL
EUR 308
Description: TAQuestâ„¢ FAST qPCR Master Mix with Helixyteâ„¢ Green is a ready-to-use 2X solution optimized for qPCR and 2-step RT-qPCR.

2X SYBR Green Fast qPCR Mix No Rox
RK21200 50mL
EUR 109

ViPrimePLUS Taq qPCR Green Master Mix II with Low ROX, 150rxns
QLMM17-LR each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix II with Low ROX, 150rxns
QLMM07-LR each Ask for price

2X SYBR Green Fast qPCR Mix with Low ROXII
RK21202 5mL
EUR 1000.08

Maxima SYBR Green/Fluorescein qPCR Master Mix - 200 x 25 µL rxns
THK0241 each Ask for price

Maxima SYBR Green/Fluorescein qPCR Master Mix - 1,000 x 25 µL rxns
THK0242 each Ask for price

Maxima SYBR Green/Fluorescein qPCR Master Mix - 4,000 x 25 µL rxns
THK0243 each Ask for price

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix (Low ROX Premixed)
Q331-03-2500rxns 2,500 rxns
EUR 562.79

Genious 2X SYBR Green Fast qPCR Mix (No ROX)
RK21205 1 ml
EUR 328.5

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix (High ROX Premixed)
Q341-03-2500rxns 2,500 rxns
EUR 562.79

rEVAlution 2X qPCR Master Mix with separate ROX
MBS308019-1000Reactions 1000Reactions
EUR 650

rEVAlution 2X qPCR Master Mix with separate ROX
MBS308019-100Reactions 100Reactions
EUR 175

rEVAlution 2X qPCR Master Mix with separate ROX
MBS308019-500Reactions 500Reactions
EUR 385

2X qPCR Probe Master Mix with Separate ROX
MBS308018-1000Reactions 1000Reactions
EUR 1150

2X qPCR Probe Master Mix with Separate ROX
MBS308018-100Reactions 100Reactions
EUR 210

2X qPCR Probe Master Mix with Separate ROX
MBS308018-2500Reactions 2500Reactions
EUR 5350

2X qPCR Probe Master Mix with Separate ROX
MBS308018-500Reactions 500Reactions
EUR 405

BioEasy Master Mix (SYBR Green)
TRI-B25L1 200T
EUR 403.2

HotStart™ 2X FAST Green qPCR Master Mix
K1171-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix
K1171-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix
K1171-50 50ml
EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

2×SYBR Green qPCR Mix (including ROX)
MBS8579841-200Tests 200Tests
EUR 285

2×SYBR Green qPCR Mix (including ROX)
MBS8579841-50Tests 50Tests
EUR 180

2×SYBR Green qPCR Mix (including ROX)
MBS8579841-5x200Tests 5x200Tests
EUR 1230

2×SYBR Green qPCR Mix (including ROX)
MBS8579841-5x50Tests 5x50Tests
EUR 770

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
Q431-03-2500rxns 2,500 rxns
EUR 656.51

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix (High ROX Premixed)
Q441-03-2500rxns 2,500 rxns
EUR 656.51

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with ROX, 150rxns
QLMM14-R each Ask for price

Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed)
RK21206 1 ml
EUR 328.5

Genious 2X SYBR Green Fast qPCR Mix (High ROX Premixed)
RK21207 1 ml
EUR 93

ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I with ROX, 150rxns
QLMM04-R each Ask for price

SG/ROX qPCR Master Mix (2x)
E0400-01 100reactions
EUR 55.59
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

SG/ROX qPCR Master Mix (2x)
E0400-02 200reactions
EUR 106.82
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

SG/ROX qPCR Master Mix (2x)
E0400-03 1000reactions
EUR 467.61
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

ABsolute SYBR Green QPCR Mix + ROX 800 rxns
AB-1162/B each Ask for price

ABsolute SYBR Green QPCR Mix + ROX 200 rxns
AB-1163 each
EUR 543.37

ChamQ SYBR qPCR Master Mix
Q311-02 500 rxns (20 μl/rxn)
EUR 120.55

ChamQ SYBR qPCR Master Mix
Q311-03 2,500 rxns (20 μl/rxn)
EUR 543.5

ChamQ SYBR qPCR Master Mix
Q311-00-100rxns 100 rxns
EUR 1.09

ChamQ SYBR qPCR Master Mix
Q311-02-500rxns 500 rxns
EUR 124.83

ChamQ SYBR qPCR Master Mix
Q311-03-2500rxns 2,500 rxns
EUR 562.79

HotStart™ Universal 2X FAST Green qPCR Master Mix
K1172-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix
K1172-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ Universal 2X FAST Green qPCR Master Mix
K1172-50 50ml
EUR 826.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

1ml PrecisionPLUS qPCR Master Mix with ROX
Z-PPLUS-R-1ML 1ml
EUR 81
Description: Precision®PLUS

ABsolute QPCR SYBR Green Low Rox Mix 800x50µl
AB-1322/B each Ask for price

ABsolute QPCR SYBR Green Low Rox Mix 200x50µl
AB-1323/A each
EUR 659.45

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix I with Low ROX, 150rxns
QLMM14-LR each Ask for price

ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix I with Low ROX, 150rxns
QLMM04-LR each Ask for price

Apex qPCR GREEN Master Mix
42-116PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, Without ROX, 5ml

Apex qPCR GREEN Master Mix
42-117PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Without ROX, 50ml

Apex qPCR GREEN Master Mix
42-118PG 400 x 25µl Reactions/Unit
EUR 338.88
Description: 2X, Low ROX, 5ml

Apex qPCR GREEN Master Mix
42-119PG 4000 x 25µl Reactions/Unit
EUR 2926.74
Description: 2X, Low ROX, 50ml

Apex qPCR GREEN Master Mix
42-120PG 400 x 25µl Reactions/Unit
EUR 341.44
Description: 2X, High ROX, 5ml

Apex qPCR GREEN Master Mix
42-121PG 4000 x 25µl Reactions/Unit
EUR 3095.57
Description: 2X, High ROX, 50ml

Fast SG/ROX qPCR Master Mix (2x)
E0413-01 100reactions
EUR 61.04
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

Fast SG/ROX qPCR Master Mix (2x)
E0413-02 200reactions
EUR 116.63
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

Fast SG/ROX qPCR Master Mix (2x)
E0413-03 1000reactions
EUR 534.1
Description: For fast-cycling quantitative real-time PCR and two-step real-time RT-PCR. Mix contains ROX dye

SG/ROX onTaq qPCR Master Mix (2x)
E0407-01 100reactions
EUR 54.5
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye

SG/ROX onTaq qPCR Master Mix (2x)
E0407-02 200reactions
EUR 104.64
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye

SG/ROX onTaq qPCR Master Mix (2x)
E0407-03 1000reactions
EUR 455.62
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Mix contains onTaq polymerase and ROX dye

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with ROX, 150rxns
QLMM18-R each Ask for price

ViPrimePLUS Taq qPCR Master Mix with ROX, 150rxns
QLMM11-R each Ask for price

ViPrimePLUS OneStep AtTaq RT-qPCR Green Master Mix II with ROX, 150rxns
QLMM08-R each Ask for price

ViPrimePLUS AtTaq qPCR Master Mix with ROX, 150rxns
QLMM01-R each Ask for price

ViPrimePLUSHigh 5X One Step RT-qPCR Green Master Mix I (SYBR® Green Dye), 100rxns
QLMM10 each Ask for price

1ml Precision FAST qPCR Master Mix with ROX
Z-PFAST-R-1ML 1ml
EUR 90
Description: Precision®FAST

2ml Precision FAST qPCR Master Mix with ROX
Z-PFAST-R-2ML 2ml
EUR 161
Description: Precision®FAST

ChamQ SYBR Color qPCR Master Mix
Q411-02 500 rxns (20 μl/rxn)
EUR 140.63

ChamQ SYBR Color qPCR Master Mix
Q411-03 2,500 rxns (20 μl/rxn)
EUR 634

ChamQ SYBR Color qPCR Master Mix
Q411-00-100rxns 100 rxns
EUR 1.09

ChamQ SYBR Color qPCR Master Mix
Q411-02-500rxns 500 rxns
EUR 145.62

ChamQ SYBR Color qPCR Master Mix
Q411-03-2500rxns 2,500 rxns
EUR 656.51

Genious 2X SYBR Green Fast qPCR Mix
RK21204 1 mL
EUR 328.5

2X Universal SYBR Green Fast qPCR Mix
RK21203 1 mL
EUR 76.5

2X Universal SYBR Green Fast qPCR Mix  
MBS9141672-25mL 25mL
EUR 755

2X Universal SYBR Green Fast qPCR Mix  
MBS9141672-5mL 5mL
EUR 255

AceQ Universal SYBR qPCR Master Mix
Q511-02 500 rxn (20 μl/rxn)
EUR 152.72

AceQ Universal SYBR qPCR Master Mix
Q511-03 2500 rxn (20 μl/rxn)
EUR 688.5

AceQ Universal SYBR qPCR Master Mix
Q511-00-100rxns 100 rxns
EUR 1.09

AceQ Universal SYBR qPCR Master Mix
Q511-02-500rxns 500 rxns
EUR 158.14

AceQ Universal SYBR qPCR Master Mix
Q511-03-2500rxns 2,500 rxns
EUR 712.94

miRNA Universal SYBR qPCR Master Mix
MQ101-01 125 rxn(20 μl/rxn)
EUR 38.9

miRNA Universal SYBR qPCR Master Mix
MQ101-02 500 rxn(20 μl/rxn)
EUR 145

ChamQ Universal SYBR qPCR Master Mix
Q711-02 500 rxn (20 μl/rxn)
EUR 152.72

ChamQ Universal SYBR qPCR Master Mix
Q711-03 2500 rxn (20 μl/rxn)
EUR 688.5

miRNA Universal SYBR qPCR Master Mix
MQ101-00-100rxns 100 rxns
EUR 1.09

miRNA Universal SYBR qPCR Master Mix
MQ101-01-125rxns 125 rxns
EUR 40.28

miRNA Universal SYBR qPCR Master Mix
MQ101-02-500rxns 500 rxns
EUR 150.15

ChamQ Universal SYBR qPCR Master Mix
Q711-00-100rxns 100 rxns
EUR 1.09

ChamQ Universal SYBR qPCR Master Mix
Q711-02-500rxns 500 rxns
EUR 158.14

ChamQ Universal SYBR qPCR Master Mix
Q711-03-2500rxns 2,500 rxns
EUR 712.94

ABsolute Blue QPCR SYBR Green ROX Mix (16x 1.25ml)
AB-4162/B each Ask for price

BrightCycle Universal SYBR Green qPCR Mix with UDG
RK21219 5mL
EUR 475

iGreen 2X qPCR Master Mix with Low-ROX, 500Rxns-20ul
A4183-4x125ML500Rxns 4x1.25ML (500Rxns)
EUR 200.2
Description: Biotechnology

ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns
QLMM12 each Ask for price

ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns
QLMM02 each Ask for price

ViPrimePLUS OneStep Taq RT-qPCR Green Master Mix II with Low ROX, 150rxns
QLMM18-LR each Ask for price

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173L 960 reactions x 20 µl
EUR 897
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain
M-PCR-173S 192 reactions x 20 µl
EUR 224
Description: lyophilised real-time PCR Master Mix with SYBR® Green fluorescent DNA stain

SG qPCR Master Mix (2x), plus ROX Solution
E0402-01 100reactions
EUR 55.59
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye

SG qPCR Master Mix (2x), plus ROX Solution
E0402-02 200reactions
EUR 106.82
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye

SG qPCR Master Mix (2x), plus ROX Solution
E0402-03 1000reactions
EUR 467.61
Description: For quantitative real-time PCR and two-step real-time RT-PCR. Contains ROX dye

MP qPCR Master Mix (2x), plus ROX Solution
E0425-01 100reactions
EUR 58.86
Description: For quantitative multiplex real-time PCR and two-step real-time RT-PCR Using sequence-specific probesContains ROX dye

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