der diagnostischen Prestatien der kommerziellen RT-PCR-Kits für die Detektion
Analysis of the diagnostic prestatien of the industrial RT-PCR kits for the detection of SARS-CoV-2 in Colombia
The ongoing SARS-CoV-2 pandemic has led to the design and development of several RT-PCR kits designed to enable rapid scale-up of molecular exams for massive screening. We evaluated the diagnostic performance of nine commercial kits that showed optimal performance and high discriminatory power. However, we observed differences in sensitivity, specificity and E-Gen-Ct values and discussed these results in light of the influence of the genetic variability of SARS-CoV-2 and its possible impact on current molecular diagnostic assays. This article is protected by copyright. All rights reserved.
Antimikrobielle Aktivität von Kamferboomzilvernanodeeltjes tegen vuilbroedziekten en het ontdekken van een nieuwe stam van Serratia marcescens mittels DGGE-PCR als Sekundärinfektion auf Honingbijlarven verabreicht wurde
Amerikanische Faulbrut (AFB) und Europäische Faulbrut (EFB) sind die beiden wichtigsten bakteriellen Krankheiten, von denen Honigbienen betroffen sind. Dies führt zu einer Verringerung der Lebensfähigkeit des Bienenstocks, einer Verringerung der Honigproduktion und zu erheblichen wirtschaftlichen Verlusten für die Imker.
Aufgrund der Ineffizienz und / oder geringen Wirksamkeit einiger Antibiotika stellen Forschungen mit Nanotechnologie möglicherweise neue therapeutische Strategien dar. Nanostruktur-Medikamente haben gegenüber herkömmlichen Medikamenten einige Vorteile gezeigt , wie langsame, schrittweise und kontrollierte Freisetzung, erhöhte Bioverfügbarkeit und verringerte Nebenwirkungen.
In dieser Studie wurden verschiedene infizierte Larven aus zwei Bienenhäusern gesammelt; Die Kämme, die Symptome einer amerikanischen und europäischen Faulbrut aufwiesen, wurden isoliert.
Die antimikrobielle In-vitro- Aktivität von Kampferbaum-Silber-Nanopartikeln gegen Faulbrutkrankheiten wurde unter Verwendung von UV-Vis-Spektrophotometrie und Rasterelektronenmikroskop (SEM) charakterisiert , was die Bildung von Silbernanopartikeln mit einem Größenbereich von 160 bis 660 nm belegt. Die antimikrobielle Aktivität der Silbernanopartikel wurde unter Verwendung eines Agardiffusionsassays getestet und bewies ihre Fähigkeit, das pathogene Bakterienwachstum sowohl in AFB als auch in EFB wirksam zu stoppen .
Die DGGE-PCR-Technik wurde zur Identifizierung seltener bakterieller Infektionen von Honigbienen in Abhängigkeit von der 16S-rRNA-Amplifikation aus ihrer gesamten extrahierten DNA angewendet und als Serratia marcescens (TES) identifiziert , die in der GenBank mit einer neuen Zugangsnummer (MT240613) hinterlegt ist.
Die Ergebnisse bestätigten, dass der Stamm durch DGGE-PCR-Analyse nachgewiesen wurde, die eine einzigartig infizierte Brut verursachte, die von der amerikanischen Foulbrood angegriffen wurde. Es konnte gefolgert werden, dass grün synthetisierte Silbernanopartikel voraussichtlich als wirksame Behandlung für bakterielle Honigbienenkrankheiten eingesetzt werden. Diese Materialien müssen unter Feldbedingungen genauer untersucht werden und untersuchen die Möglichkeit ihrer Rückstände in Honigbienen wie Honig und Bienenwachs.
Recombinant Human Relaxin-3 |
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| MBS140915-1mg | MyBiosource | 1mg | EUR 2880 |
Recombinant Human Relaxin-3 |
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| MBS140915-5x1mg | MyBiosource | 5x1mg | EUR 12630 |
Relaxin-3, human recombinant |
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| 7195-10 | Biovision | each | EUR 248.4 |
Relaxin-3, human recombinant |
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| 7195-50 | Biovision | each | EUR 810 |
Relaxin-3 Human Recombinant |
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| rAP-4650 | Angio Proteomie | Inquiry | Ask for price |
INSL7 / Relaxin 3 (Human) |
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| 035-36 | PHOENIX PEPTIDE | 20 μg | EUR 160.92 |
INSL7 / Relaxin 3 (Human) |
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| 035-36A | PHOENIX PEPTIDE | 100 μg | EUR 604.8 |
Human Relaxin-3 ELISA Kit |
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| MBS2887639-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 4080 |
Human Relaxin-3 ELISA Kit |
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| MBS2887639-48StripWells | MyBiosource | 48-Strip-Wells | EUR 390 |
Human Relaxin-3 ELISA Kit |
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| MBS2887639-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2220 |
Human Relaxin-3 ELISA Kit |
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| MBS2887639-96StripWells | MyBiosource | 96-Strip-Wells | EUR 520 |
Human Relaxin-3 ELISA Kit |
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| MBS765502-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3900 |
Human Relaxin-3 ELISA Kit |
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| MBS765502-48StripWells | MyBiosource | 48-Strip-Wells | EUR 340 |
Human Relaxin-3 ELISA Kit |
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| MBS765502-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2045 |
Human Relaxin-3 ELISA Kit |
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| MBS765502-96StripWells | MyBiosource | 96-Strip-Wells | EUR 455 |
Human Relaxin-3,RLN-3 ELISA |
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| YLA4309HU-48T | Shanghai YL Biotech | 48T | Ask for price |
Human Relaxin-3,RLN-3 ELISA |
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| YLA4309HU-96T | Shanghai YL Biotech | 96T | Ask for price |
INSL7 / Relaxin 3 (Human) - Antibody |
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| H-035-36 | PHOENIX PEPTIDE | 50 μl | EUR 604.8 |
Human Relaxin 3 ELISA Kit |
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| MBS054925-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 6725 |
Human Relaxin 3 ELISA Kit |
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| MBS054925-48StripWells | MyBiosource | 48-Strip-Wells | EUR 550 |
Human Relaxin 3 ELISA Kit |
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| MBS054925-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3420 |
Human Relaxin 3 ELISA Kit |
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| MBS054925-96StripWells | MyBiosource | 96-Strip-Wells | EUR 765 |
Human Relaxin 3 ELISA Kit |
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| EK5653 | SAB | 96 tests | EUR 599 |
Human Relaxin 3 ELISA Kit |
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| MBS8248392-5x96Tests | MyBiosource | 5x96Tests | EUR 2880 |
Human Relaxin 3 ELISA Kit |
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| MBS8248392-96Tests | MyBiosource | 96Tests | EUR 645 |
Human relaxin 3 ELISA Kit |
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| SL2396Hu | Sunlong | 96 Tests | EUR 468 |
Mouse Monoclonal anti-human Relaxin-3 |
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| hAP-0526 | Angio Proteomie | 100ug | EUR 250 |
Human Relaxin-3 Recombinant Protein |
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| 100-356 | ReliaTech | 25 µg | EUR 196.35 |
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Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
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Human Relaxin-3 Recombinant Protein |
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| 100-356S | ReliaTech | 5 µg | EUR 92.4 |
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Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
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Recombinant Human Relaxin-3 Protein |
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| PROTQ8WXF3-1 | BosterBio | 25ug | EUR 380.4 |
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Description: Relaxin-3 (H3 relaxin, Insulin-like peptide-7, INSL7) is a secreted protein structurally related to insulin, which is expressed primarily in the brain and central nervous system. Relaxin-3 has been identified as the ligand for the GPCR135 receptor, previously known as “somatostatin-like” or “angiotensin-like” peptide receptor, and also binds specifically to the LGR7 receptor, previously identified as an “orphan” G protein coupled receptor. Signaling by Relaxin-3 through its target receptors is, most likely, part of a CNS processing system, activated in response to signaling by neuropeptides and other factors. Intracerebroventricular injections of Relaxin-3 have been shown to cause a significant increase of food intake and body weight in Wistar rats. Recombinant Relaxin-3 is a 5.5 kDa disulfide linked heterodimeric protein consisting of a 24 amino acid A-chain and a 27 amino acid B-chain. |
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Human Relaxin-1 |
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| hAP-0525 | Angio Proteomie | 50ug | EUR 400 |
Human Relaxin-2 |
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| MBS691734-0005mg | MyBiosource | 0.005mg | EUR 300 |
Human Relaxin-2 |
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| MBS691734-0025mg | MyBiosource | 0.025mg | EUR 450 |
Human Relaxin-2 |
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| MBS691734-5x0025mg | MyBiosource | 5x0.025mg | EUR 1725 |
Human Relaxin 3 (RLN3) Protein |
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| abx680520-100g | Abbexa | 100 µg | EUR 225 |
ELISA Kit for Human Relaxin-3 |
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| E0189h | EIAab | 96T | EUR 387.4 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| AE22052HU-48T | Abebio | 48T | EUR 325 |
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Description: Human (Homo sapiens) |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| AE22052HU-48Tests | Abebio | 48 Tests | EUR 325 |
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Description: Human (Homo sapiens) |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| AE22052HU-96T | Abebio | 96T | EUR 610 |
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Description: Human (Homo sapiens) |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| AE22052HU-96Tests | Abebio | 96 Tests | EUR 610 |
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Description: Human (Homo sapiens) |
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Human Relaxin-3(RLN3) Elisa Kit |
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| EK713553 | AFG Bioscience LLC | 96 Wells | EUR 0.37 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| EK7514 | SAB | 96Т | EUR 799 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| KTE60790-48T | Abbkine | 48T | EUR 339 |
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Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| KTE60790-96T | Abbkine | 96T | EUR 589 |
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Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| KTE60790-96T5 | Abbkine | 96 T×5 | EUR 2629 |
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Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| KTE60790-96T50 | Abbkine | 96 T×50 | EUR 24569 |
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Description: This Human Relaxin-3 (RLN3) ELISA Kit employs a two-site sandwich ELISA to quantitate RLN3. |
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Human Relaxin-3 (RLN3) ELISA Kit |
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| MBS9714455-48Tests | MyBiosource | 48Tests | EUR 340 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| MBS9714455-5x96Tests | MyBiosource | 5x96Tests | EUR 1820 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| MBS9714455-96Tests | MyBiosource | 96Tests | EUR 500 |
Human Relaxin-3 (RLN3) ELISA Kit |
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| RK06347 | Abclonal | 96T | EUR 322.37 |
Relaxin-2 (Human) |
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| MBS408061-005mg | MyBiosource | 0.05mg | EUR 345 |
Relaxin-2 (Human) |
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| MBS408061-5x005mg | MyBiosource | 5x0.05mg | EUR 1500 |
Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7) |
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| MBS650130-0005mg | MyBiosource | 0.005mg | EUR 340 |
Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7) |
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| MBS650130-0025mg | MyBiosource | 0.025mg | EUR 490 |
Relaxin 3, Recombinant, Human (H3 Relaxin, Insulin-Like Peptide-7, INSL7) |
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| MBS650130-5x0025mg | MyBiosource | 5x0.025mg | EUR 2055 |
Human RLN3/ Relaxin-3 ELISA Kit |
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| E2158Hu | Sunlong | 1 Kit | EUR 515 |
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Description: UNQ6188/PRO20213,INSL7,RXN3,ZINS4,Insulin-like peptide INSL7,Prorelaxin H3,Insulin-like peptide 7 |
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Human RLN3(Relaxin-3) ELISA Kit |
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| EH0755 | FN Test | 96T | EUR 681.12 |
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Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
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Human RLN3 (Relaxin-3) ELISA Kit |
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| MBS2516020-10x96Tests | MyBiosource | 10x96Tests | EUR 4060 |
Human RLN3 (Relaxin-3) ELISA Kit |
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| MBS2516020-24Tests | MyBiosource | 24Tests | EUR 265 |
Human RLN3 (Relaxin-3) ELISA Kit |
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| MBS2516020-48Test | MyBiosource | 48Test | EUR 450 |
Human RLN3 (Relaxin-3) ELISA Kit |
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| MBS2516020-5x96Test | MyBiosource | 5x96Test | EUR 2265 |
Human RLN3 (Relaxin-3) ELISA Kit |
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| MBS2516020-96Tests | MyBiosource | 96Tests | EUR 535 |
Human RLN3(Relaxin-3) ELISA Kit |
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| RE1961H-48wells | Reed Biotech | 48 wells | EUR 125.55 |
Human RLN3(Relaxin-3) ELISA Kit |
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| RE1961H-48wellsplate | Reed Biotech | 48 wells plate | EUR 126 |
Human RLN3(Relaxin-3) ELISA Kit |
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| RE1961H-96wells | Reed Biotech | 96 wells | EUR 179.55 |
Human RLN3(Relaxin-3) ELISA Kit |
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| RE1961H-96wellsplate | Reed Biotech | 96 wells plate | EUR 180 |
Human Relaxin 3 (RLN3) ELISA Kit |
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| abx512123-96tests | Abbexa | 96 tests | EUR 904.8 |
Human Relaxin 3 (RLN3) ELISA Kit |
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| abx253713-96tests | Abbexa | 96 tests | EUR 562.5 |
Human Relaxin 3,RLN3 ELISA KIT |
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| JOT-EK7543Hu | Jotbody | 96 wells | Ask for price |
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Description: Human |
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Human Relaxin 3,RLN3 ELISA KIT |
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| E7543Hu-1096T | Jiaxing Korain Biotech Ltd (BT Labs) | 10*96T | EUR 4122 |
Human Relaxin 3,RLN3 ELISA KIT |
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| E7543Hu-48wells | Jiaxing Korain Biotech Ltd (BT Labs) | 48 wells | EUR 300 |
Human Relaxin 3,RLN3 ELISA KIT |
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| E7543Hu-596T | Jiaxing Korain Biotech Ltd (BT Labs) | 5*96T | EUR 2061 |
Human Relaxin 3,RLN3 ELISA KIT |
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| E7543Hu-96wells | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 458 |
Human Relaxin 3, RLN3 ELISA Kit |
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| MBS1609027-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3460 |
Human Relaxin 3, RLN3 ELISA Kit |
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| MBS1609027-48StripWells | MyBiosource | 48-Strip-Wells | EUR 285 |
Human Relaxin 3, RLN3 ELISA Kit |
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| MBS1609027-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1750 |
Human Relaxin 3, RLN3 ELISA Kit |
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| MBS1609027-96StripWells | MyBiosource | 96-Strip-Wells | EUR 425 |
Nori® Human Relaxin-3 ELISA Kit |
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| GR111288 | Genorise Scientific | 96-well | EUR 461 |
Relaxin 2 (Human) |
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| 035-62 | PHOENIX PEPTIDE | 20 μg | EUR 261.36 |
Relaxin 2 (Human) |
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| 035-62A | PHOENIX PEPTIDE | 100 μg | EUR 469.8 |
Relaxin H2 (human) |
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| 4039910.02 | Bachem | 0.2 mg | EUR 455.07 |
Relaxin H2 (human) |
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| 4039910.1 | Bachem | 1 mg | EUR 1818.92 |
Relaxin H3 (human) |
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| 4039569.02 | Bachem | 0.2 mg | EUR 455.07 |
Relaxin H3 (human) |
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| 4039569.1 | Bachem | 1 mg | EUR 1818.92 |
Relaxin H2 (human) |
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| H-6784.0200 | Bachem | 0.2mg | EUR 741.6 |
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Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net |
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Relaxin H2 (human) |
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| H-6784.1000 | Bachem | 1.0mg | EUR 2853.6 |
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Description: Sum Formula: C256H408N74O74S8; CAS# [99489-94-8] net |
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Relaxin H3 (human) |
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| H-7322.0200 | Bachem | 0.2mg | EUR 741.6 |
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Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net |
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Relaxin H3 (human) |
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| H-7322.1000 | Bachem | 1.0mg | EUR 2853.6 |
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Description: Sum Formula: C237H374N70O69S6; CAS# [1158181-62-4] net |
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Recombinant Human Relaxin-3 (RLN3), partial |
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| MBS7094488-005mgBaculovirus | MyBiosource | 0.05mg(Baculovirus) | EUR 885 |
Recombinant Human Relaxin-3 (RLN3), partial |
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| MBS7094488-005mgEColi | MyBiosource | 0.05mg(E-Coli) | EUR 510 |
Recombinant Human Relaxin-3 (RLN3), partial |
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| MBS7094488-02mgEColi | MyBiosource | 0.2mg(E-Coli) | EUR 665 |
Recombinant Human Relaxin-3 (RLN3), partial |
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| MBS7094488-02mgYeast | MyBiosource | 0.2mg(Yeast) | EUR 945 |
Recombinant Human Relaxin-3 (RLN3), partial |
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| MBS7094488-05mgEColi | MyBiosource | 0.5mg(E-Coli) | EUR 710 |
ELISA kit for Human Relaxin-3 (RLN3) |
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| KTE60790-5platesof96wells | Abbkine | 5 plates of 96 wells | EUR 2538 |
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Description: Quantitative sandwich ELISA for measuring Human Relaxin-3 (RLN3) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
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INSL7 / Relaxin 3 (Human) - RIA Kit |
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| RK-035-36 | PHOENIX PEPTIDE | 125 tubes | EUR 1061.64 |
Relaxin 2 (Human) - Antibody |
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| H-035-62 | PHOENIX PEPTIDE | 100 μl | EUR 604.8 |
Relaxin-3 |
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| AP87248 | SAB | 1mg | EUR 2640 |
Relaxin-3 |
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| AP87370 | SAB | 1mg | EUR 2640 |
Relaxin-3 |
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| AP87734 | SAB | 1mg | EUR 2640 |
Relaxin-3 |
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| AP88315 | SAB | 1mg | EUR 2640 |
Human Relaxin 3-RLN3-ELISA Kit |
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| QY-E01376 | Qayee Biotechnology | 96T | EUR 400 |
Human RLN3 (Relaxin 3) ELISA Kit |
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| MBS8819812-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3130 |
Human RLN3 (Relaxin 3) ELISA Kit |
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| MBS8819812-48StripWells | MyBiosource | 48-Strip-Wells | EUR 350 |
Human RLN3 (Relaxin 3) ELISA Kit |
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| MBS8819812-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1710 |
Human RLN3 (Relaxin 3) ELISA Kit |
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| MBS8819812-96StripWells | MyBiosource | 96-Strip-Wells | EUR 445 |
Human Relaxin 3 PicoKine ELISA Kit |
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| MBS177627-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3935 |
Human Relaxin 3 PicoKine ELISA Kit |
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| MBS177627-96StripWells | MyBiosource | 96-Strip-Wells | EUR 855 |
RLN3 (untagged)-Human relaxin 3 (RLN3) |
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| SC305868 | Origene Technologies GmbH | 10 µg | Ask for price |
Anti- Relaxin-3 receptor 2 Antibody |
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| GWB-EDDDF9 | GenWay Biotech | 0.05 ml | Ask for price |
Anti- Relaxin-3 receptor 2 Antibody |
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| GWB-214500 | GenWay Biotech | 0.05 ml | Ask for price |
ELISA kit for Human RLN3 (Relaxin-3) |
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| E-EL-H1652 | Elabscience Biotech | 1 plate of 96 wells | EUR 640.8 |
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Description: A sandwich ELISA kit for quantitative measurement of Human RLN3 (Relaxin-3) in samples from Serum, Plasma, Cell supernatant |
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Relaxin 3 (BDelta23-27) R (Human) |
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| 036-20 | PHOENIX PEPTIDE | 100 μg | EUR 461.16 |
EasyPro Human Relaxin-3(RLN3)ELISA Kit |
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| FY-EH1961S | Feiyuebio | each | EUR 520 |
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Description: 18.75 pg/mL |
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RLN3 Relaxin-3 Human Recombinant Protein |
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| PROTQ8WXF3 | BosterBio | Regular: 25ug | EUR 250 |
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Description: Relaxin-3 Human Recombinant produced in E. coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain. The Relaxin-3 is purified by proprietary chromatographic techniques. |
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INSL7 / Relaxin 3 (Human) - Cy5 Labeled |
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| FC5-035-36 | PHOENIX PEPTIDE | 1 nmol | EUR 756 |
Human Relaxin-3 Recombinant Protein Lyophilized |
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| IHURLXN3RLY5UG | Innovative research | each | EUR 341 |
|
Description: Human Relaxin-3 Recombinant Protein Lyophilized |
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Human Relaxin-3 Recombinant Protein Lyophilized |
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| MBS8433281-INQUIRE | MyBiosource | INQUIRE | Ask for price |
INSL7 / Relaxin 3 (Human) - Purified IgG Antibody |
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| G-035-36 | PHOENIX PEPTIDE | 200 μg | EUR 604.8 |
Recombinant Human Relaxin-2 |
|||
| MBS146516-0005mg | MyBiosource | 0.005mg | EUR 240 |
Recombinant Human Relaxin-2 |
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| MBS146516-0025mg | MyBiosource | 0.025mg | EUR 310 |
Een zeer gevoelige en specifieke sonde-gebaseerde Echtzeit-PCR für den Nachweis von Avibacterium paragallinarum in klinischen Monstern van pluimvee
Avibacterium paragallinarum (historisch genannt Haemophilus paragallinarum ) verursacht infektiöse Coryza (IC), die eine akute respiratorische Erkrankung von Hühnern ist. Kürzlich wurden in Pennsylvania (PA) Ausbrüche von IC bei Broilern, Legehennen und Legehennen gemeldet, die zu erheblichen Atemwegserkrankungen und Produktionsverlusten führten. Eine vorläufige Diagnose des IC kann auf der Grundlage der Anamnese, der klinischen Symptome und der charakteristischen groben Läsionen gestellt werden. Für eine endgültige Diagnose sind jedoch eine Isolierung und Identifizierung des Organismus erforderlich. Große Herausforderungen bei der bakteriologischen Diagnose von A. paragallinarum, gehört, dass der Organismus schwer zu isolieren ist, langsam wächst und nur erfolgreich sein kann Im akuten Stadium der Infektion isolierte und sekundäre bakterielle Infektionen sind ebenfalls häufig.
Da die öffentlichen Datenbanken nur sehr begrenzte Gesamtgenome von A. paragallinarum enthielten , führten wir eine Gesamtgenomsequenzierung (WGS) von PA-Isolaten durch und basierten auf der WGS-Datenanalyse. Wir haben einen neuartigen sondenbasierten PCR-Assay entwickelt, der auf eine hochkonservierte Sequenz im recN , dem DNA-Reparaturprotein-Gen von A. paragallinarum, abzielt . Der Assay umfasst eine interne Kontrolle mit einer Nachweisgrenze (LOD) von 3,93 genomischen Kopien. Die PCR-Effizienz lag zwischen 90 und 97% und die diagnostische Sensitivität von 98,5% im Vergleich zur herkömmlichen gelbasierten PCR.
Der Take a look at battle hochspezifisch und es wurde keine Kreuzreaktivität mit anderen Avibacterium- Arten und einer Reihe anderer üblicher viraler und bakterieller Pathogene der Atemwege von Geflügel beobachtet . Echtzeit-PCR-Exams an 419 klinischen Proben aus vermuteten Herden ergaben 94 optimistic und 365 adverse Ergebnisse in Übereinstimmung mit dem diagnostischen Nachweis auf der Foundation von Bakterienkulturen. Wir verglichen auch den recN- PCR- Assay mit einem früheren HPG-2- basierten Echtzeit-PCR-Assay, der eine PCR-Effizienz von 79% zeigte.
Gelijktijdige detectie en differentiatie van klinisch relevante recidiverende koorts Borrelia erfüllt semi-Multiplex real-time PCR
Ailments transmitted by bacterial vectors, together with Borrelia species, pose a big diagnostic, medical, and well being problem due to their overlapping signs and the breadth of pathogens and arthropod vectors. Recurrent fever borrelia (RF) consists of each established and rising pathogens are transmitted to people by gentle ticks, onerous ticks or lice.
We’ve developed a real-time, semi-multiplexed PCR assay that detects a number of RF borrelia, which trigger illness in people, and divides them into one among three teams.
The teams are primarily based on genetic similarity and embody pathogens inflicting relapsing fever in gentle ticks ( B. hermsii and others), the rising onerous tick-borne pathogen B. miyamotoi, and the pathogen accountable for relapsing fever ( B. recentis) . Assay makes use of a single primer pair designed to amplify all recognized RF pathogenic borrelia and a number of TaqMan probes to allow detection and differentiation between the three teams.
The assay acknowledges all examined RF Borrelia with an analytical detection restrict of lower than 15 genome equivalents per response. Thirty isolates of RF Borrelia spanning six species have been precisely recognized. 39 of 41 residual samples (EDTA entire blood , serum or plasma) from sufferers with RF had been detected and accurately categorized.
None of 42 medical specimens from sufferers with different infections and 46 tradition specimens from non-RF micro organism had been detected. Creating a real-time PCR method to a single assay will assist enhance the prognosis of RF by simplifying the choice of assessments to help the medical administration of acutely unwell RF sufferers.
Overlevingsanalyse van de tijd tot SARS-CoV-2 PCR-Negativierung om PCR-Receptor bij gezondheidswerkers te optimaliseren: de Henares COVID-19 cohort research voor gezondheidswerkers
Goals: Reverse transcriptase PCR (RT-PCR) is taken into account the gold commonplace in diagnosing COVID-19. Contaminated well being care staff won’t return to work till RT-PCR exhibits that the virus is now not current within the higher respiratory tract. The goal of this research is to find out essentially the most environment friendly time to carry out an RT-PCR previous to reintegrating healthcare staff.
Supplies and Strategies: This can be a cohort research of staff of well being care with RT-PCR confirmed COVID-19th The info was collected from the well being care staff‘ medical charts and accomplished with a phone interview. Kaplan-Meier curves had been used to find out the affect of a number of variables on the time to RT-PCR negatization. The affect of the variables on survival was assessed utilizing the Breslow check. A Cox regression mannequin was developed containing the related variables.
Outcomes: 159 topics with a optimistic RT-PCR from 374 staff with suspected COVID-19 had been included. The median time to negatization was 25 days from the onset of signs (IQR 20-35 days). The presence of IgG, dyspnoea, cough, and sore throat had been related to a considerably longer time to negatization. Cox logistic regression was used to regulate for complicated variables. Solely dyspnoea and cough remained within the mannequin as important determinants for a chronic negativity time. The adjusted HRs had been 0.68 (0.48-096) for dyspnea and 0.61 (0.42-0.88) for dry cough.
Conclusions: RT-PCR within the first Three weeks results in a excessive share of optimistic outcomes. If there have been respiratory issues, the negatization lasted virtually per week longer. Those that developed antibodies took longer to negate.
SYBR Green qPCR Master Mix (No ROX)
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Vazyme
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17271
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17271-5mL
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17272
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17272-1mL
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17273
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17273-5mL
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17274
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17274-1mL
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17275
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17275-5mL
AAT Bioquest
5 mL
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TRI Biotech
200T
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17276
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17276-1mL
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17277
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17277-5mL
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17278
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17278-1mL
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17279
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17279-5mL
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17280
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17280-1mL
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17281
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17281-5mL
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ApexBio
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RK21205
Abclonal
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Vazyme
500 rxns (20 μl/rxn)
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Vazyme
500 rxns
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Q341-03
Vazyme
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MyBiosource
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EnoGene
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EnoGene
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MyBiosource
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MyBiosource
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MyBiosource
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TRI-B25L1
TRI Biotech
200T
EUR 403.2
HotStart™ 2X FAST Green qPCR Master Mix
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ApexBio
25ml
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K1171-5
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K1171-50
ApexBio
50ml
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ChamQ SYBR Color qPCR Master Mix (Low ROX Premixed)
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Q431-02-500rxns
Vazyme
500 rxns
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Q431-03
Vazyme
2,500 rxns (20 μl/rxn)
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Vazyme
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500 rxns (20 μl/rxn)
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Q441-02-500rxns
Vazyme
500 rxns
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Q441-03
Vazyme
2,500 rxns (20 μl/rxn)
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Q441-03-2500rxns
Vazyme
2,500 rxns
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HotStart™ Universal 2X Green qPCR Master Mix
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ApexBio
25ml
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K1170-5
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K1170-50
ApexBio
50ml
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Genious 2X SYBR Green Fast qPCR Mix (Low ROX Premixed)
RK21206
Abclonal
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1000reactions
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Signosis
2 ml
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4302A
Unibiotest
1 mL
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Unibiotest
100*1 mL
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EnoGene Green qPCR Master Mix
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EnoGene
500 reaction
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Vazyme
2,500 rxns
EUR 562.79
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Vazyme
100 rxns
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Q311-02
Vazyme
500 rxns (20 μl/rxn)
EUR 120.55
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Q311-02-500rxns
Vazyme
500 rxns
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Q311-03
Vazyme
2,500 rxns (20 μl/rxn)
EUR 543.5
HotStart™ Universal 2X FAST Green qPCR Master Mix
K1172-25
ApexBio
25ml
EUR 424
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K1172-5
ApexBio
5ml
EUR 132
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K1172-50
ApexBio
50ml
EUR 826.4
1ml PrecisionPLUS qPCR Master Mix with ROX
Z-PPLUS-R-1ML
Novacyt Group
1ml
EUR 81
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AB-1322/B
Westburg
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Genesee Scientific
400 x 25µl Reactions/Unit
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42-117PG
Genesee Scientific
4000 x 25µl Reactions/Unit
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Apex qPCR GREEN Master Mix
42-118PG
Genesee Scientific
400 x 25µl Reactions/Unit
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42-119PG
Genesee Scientific
4000 x 25µl Reactions/Unit
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Apex qPCR GREEN Master Mix
42-120PG
Genesee Scientific
400 x 25µl Reactions/Unit
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42-121PG
Genesee Scientific
4000 x 25µl Reactions/Unit
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E0413-01
EURx
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E0413-02
EURx
200reactions
EUR 116.63
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E0413-03
EURx
1000reactions
EUR 534.1
SG/ROX onTaq qPCR Master Mix (2x)
E0407-01
EURx
100reactions
EUR 54.5
SG/ROX onTaq qPCR Master Mix (2x)
E0407-02
EURx
200reactions
EUR 104.64
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E0407-03
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Vivantis
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QLMM10
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1ml Precision FAST qPCR Master Mix with ROX
Z-PFAST-R-1ML
Novacyt Group
1ml
EUR 90
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Z-PFAST-R-2ML
Novacyt Group
2ml
EUR 161
Genious 2X SYBR Green Fast qPCR Mix
RK21204
Abclonal
1 mL
EUR 76.3
ChamQ SYBR Color qPCR Master Mix
Q411-00-100rxns
Vazyme
100 rxns
EUR 1.09
ChamQ SYBR Color qPCR Master Mix
Q411-02
Vazyme
500 rxns (20 μl/rxn)
EUR 140.63
ChamQ SYBR Color qPCR Master Mix
Q411-02-500rxns
Vazyme
500 rxns
EUR 145.62
ChamQ SYBR Color qPCR Master Mix
Q411-03
Vazyme
2,500 rxns (20 μl/rxn)
EUR 634
ChamQ SYBR Color qPCR Master Mix
Q411-03-2500rxns
Vazyme
2,500 rxns
EUR 656.51
2X Universal SYBR Green Fast qPCR Mix
MBS9141672-25mL
MyBiosource
25mL
EUR 755
2X Universal SYBR Green Fast qPCR Mix
MBS9141672-5mL
MyBiosource
5mL
EUR 255
2X Universal SYBR Green Fast qPCR Mix
RK21203
Abclonal
1 mL
EUR 21.26
AceQ Universal SYBR qPCR Master Mix
Q511-00-100rxns
Vazyme
100 rxns
EUR 1.09
AceQ Universal SYBR qPCR Master Mix
Q511-02
Vazyme
500 rxn (20 μl/rxn)
EUR 152.72
AceQ Universal SYBR qPCR Master Mix
Q511-02-500rxns
Vazyme
500 rxns
EUR 158.14
AceQ Universal SYBR qPCR Master Mix
Q511-03
Vazyme
2500 rxn (20 μl/rxn)
EUR 688.5
AceQ Universal SYBR qPCR Master Mix
Q511-03-2500rxns
Vazyme
2,500 rxns
EUR 712.94
ChamQ Universal SYBR qPCR Master Mix
Q711-00-100rxns
Vazyme
100 rxns
EUR 1.09
ChamQ Universal SYBR qPCR Master Mix
Q711-02
Vazyme
500 rxn (20 μl/rxn)
EUR 152.72
ChamQ Universal SYBR qPCR Master Mix
Q711-02-500rxns
Vazyme
500 rxns
EUR 158.14
ChamQ Universal SYBR qPCR Master Mix
Q711-03
Vazyme
2500 rxn (20 μl/rxn)
EUR 688.5
ChamQ Universal SYBR qPCR Master Mix
Q711-03-2500rxns
Vazyme
2,500 rxns
EUR 712.94
miRNA Universal SYBR qPCR Master Mix
MQ101-00-100rxns
Vazyme
100 rxns
EUR 1.09
miRNA Universal SYBR qPCR Master Mix
MQ101-01
Vazyme
125 rxn(20 μl/rxn)
EUR 38.9
miRNA Universal SYBR qPCR Master Mix
MQ101-01-125rxns
Vazyme
125 rxns
EUR 40.28
miRNA Universal SYBR qPCR Master Mix
MQ101-02
Vazyme
500 rxn(20 μl/rxn)
EUR 145
miRNA Universal SYBR qPCR Master Mix
MQ101-02-500rxns
Vazyme
500 rxns
EUR 150.15
ABsolute Blue QPCR SYBR Green ROX Mix (16x 1.25ml)
AB-4162/B
Westburg
each
Ask for price
BrightCycle Universal SYBR Green qPCR Mix with UDG
RK21219
Abclonal
5mL
EUR 29.98
iGreen 2X qPCR Master Mix with Low-ROX, 500Rxns-20ul
A4183-4x125ML500Rxns
Biomatik Corporation
4x1.25ML (500Rxns)
EUR 200.2
ViPrimePLUS Taq qPCR Green Master Mix I, 150rxns
QLMM12
Vivantis
each
Ask for price
ViPrimePLUS AtTaq qPCR Green Master Mix I, 150rxns
QLMM02
Vivantis
each
Ask for price
Kaninchen-Ergänzung UnadsoRabbited 100 ml
Plazenta-Analysen und diagnostische Analysen, Beperkingen Suggereren Dat Verticale Transmissie
toepassingen van de PCR Einzelstrang-Konformations-Polymorphismus-Methode
Über den Autor
